The Jacksonville Brothel was a brothel located in Jacksonville, Florida.
The brothel was run by Aunt Ruby while she raised teenage Bobbie and Luke, Bobbie’s brother. When his sister was with clients, Luke would take their wallets and later ran scams.
In June 2011, Lucky tracks down Luke to the Jacksonville brothel but Luke is with the prostitute Lupe and cruelly rejects him. When Lucky returns to Port Charles and reports this to his family, Lulu begins to give up hope that she will reunite with her father. Yet when Lucky burns down the Spencer House that Lulu was raised in, she decides that she must retrieve her father herself, for Lucky's sake, and departs for Florida.
By the time Lulu arrives at the brothel, Luke has apparently left. Posing as his former business associate, Lulu questions the dangerous brothel manager Javier and Lupe, the prostitute who was with Luke. The two hint that Luke might return, so Lulu takes a job as a cocktail waitress in the brothel with hopes to reunite with him soon. Dante follows her and pretends not to know her, then accepts a job from Javier as a bouncer. Soon Javier learns of the pair's deceit, and kills Lupe when he believes she is giving them information. He tells Dante and Lulu that Lupe was drunk and she slipped in the shower and broke her neck.
Javier has his thugs beat and tie up Dante while he tries to rape Lulu, but Dante escapes and saves her, overpowering Javier. At gunpoint, Javier confesses that he knows Luke is somewhere doing business with his old nemesis, Nikolas' grandmother Helena Cassadine. Dante arrests Javier and leaves him in cuffs for the local authorities.
Lulu and Dante then burn down the brothel and travel to Greece to find Luke and Helena.